A high ATP concentration enhances the cooperative translocation of the SARS coronavirus helicase nsP13 in the unwinding of duplex RNA
Identifieur interne : 000A35 ( 2020/Analysis ); précédent : 000A34; suivant : 000A36A high ATP concentration enhances the cooperative translocation of the SARS coronavirus helicase nsP13 in the unwinding of duplex RNA
Auteurs : Kyoung-Jin Jang [Corée du Sud] ; Seonghwan Jeong [Corée du Sud] ; Dong Young Kang [Corée du Sud] ; Nipin Sp [Corée du Sud] ; Young Mok Yang [Corée du Sud] ; Dong-Eun Kim [Corée du Sud]Source :
- Scientific Reports [ 2045-2322 ] ; 2020.
Abstract
Severe acute respiratory syndrome coronavirus nonstructural protein 13 (SCV nsP13), a superfamily 1 helicase, plays a central role in viral RNA replication through the unwinding of duplex RNA and DNA with a 5′ single-stranded tail in a 5′ to 3′ direction. Despite its putative role in viral RNA replication, nsP13 readily unwinds duplex DNA by cooperative translocation. Herein, nsP13 exhibited different characteristics in duplex RNA unwinding than that in duplex DNA. nsP13 showed very poor processivity on duplex RNA compared with that on duplex DNA. More importantly, nsP13 inefficiently unwinds duplex RNA by increasing the 5′-ss tail length. As the concentration of nsP13 increased, the amount of unwound duplex DNA increased and that of unwound duplex RNA decreased. The accumulation of duplex RNA/nsP13 complexes increased as the concentration of nsP13 increased. An increased ATP concentration in the unwinding of duplex RNA relieved the decrease in duplex RNA unwinding. Thus, nsP13 has a strong affinity for duplex RNA as a substrate for the unwinding reaction, which requires increased ATPs to processively unwind duplex RNA. Our results suggest that duplex RNA is a preferred substrate for the helicase activity of nsP13 than duplex DNA at high ATP concentrations.
Url:
DOI: 10.1038/s41598-020-61432-1
PubMed: 32161317
PubMed Central: 7066239
Affiliations:
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Dong Eun" sort="Kim, Dong Eun" uniqKey="Kim D" first="Dong-Eun" last="Kim">Dong-Eun Kim</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0532 8339</institution-id><institution-id institution-id-type="GRID">grid.258676.8</institution-id><institution>Department of Bioscience and Biotechnology,</institution><institution>Konkuk University,</institution></institution-wrap>Seoul, 05029 Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Seoul</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Severe acute respiratory syndrome coronavirus nonstructural protein 13 (SCV nsP13), a superfamily 1 helicase, plays a central role in viral RNA replication through the unwinding of duplex RNA and DNA with a 5′ single-stranded tail in a 5′ to 3′ direction. Despite its putative role in viral RNA replication, nsP13 readily unwinds duplex DNA by cooperative translocation. Herein, nsP13 exhibited different characteristics in duplex RNA unwinding than that in duplex DNA. nsP13 showed very poor processivity on duplex RNA compared with that on duplex DNA. More importantly, nsP13 inefficiently unwinds duplex RNA by increasing the 5′-ss tail length. As the concentration of nsP13 increased, the amount of unwound duplex DNA increased and that of unwound duplex RNA decreased. The accumulation of duplex RNA/nsP13 complexes increased as the concentration of nsP13 increased. An increased ATP concentration in the unwinding of duplex RNA relieved the decrease in duplex RNA unwinding. Thus, nsP13 has a strong affinity for duplex RNA as a substrate for the unwinding reaction, which requires increased ATPs to processively unwind duplex RNA. Our results suggest that duplex RNA is a preferred substrate for the helicase activity of nsP13 than duplex DNA at high ATP concentrations.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Marra, Ma" uniqKey="Marra M">MA Marra</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rota, Pa" uniqKey="Rota P">PA Rota</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J Ziebuhr</name></author><author><name sortKey="Snijder, Ej" uniqKey="Snijder E">EJ Snijder</name></author><author><name sortKey="Gorbalenya, Ae" uniqKey="Gorbalenya A">AE Gorbalenya</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brierley, I" uniqKey="Brierley I">I Brierley</name></author><author><name sortKey="Digard, P" uniqKey="Digard P">P Digard</name></author><author><name sortKey="Inglis, Sc" uniqKey="Inglis 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uniqKey="Seybert A">A Seybert</name></author><author><name sortKey="Hegyi, A" uniqKey="Hegyi A">A Hegyi</name></author><author><name sortKey="Siddell, Sg" uniqKey="Siddell S">SG Siddell</name></author><author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J Ziebuhr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kawaoka, J" uniqKey="Kawaoka J">J Kawaoka</name></author><author><name sortKey="Pyle, Am" uniqKey="Pyle A">AM Pyle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mackintosh, Sg" uniqKey="Mackintosh S">SG Mackintosh</name></author><author><name sortKey="Raney, Kd" uniqKey="Raney K">KD Raney</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ozdemir, Ay" uniqKey="Ozdemir A">AY Ozdemir</name></author><author><name sortKey="Rusanov, T" uniqKey="Rusanov T">T Rusanov</name></author><author><name sortKey="Kent, T" uniqKey="Kent T">T Kent</name></author><author><name 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sortKey="Sugimoto, N" uniqKey="Sugimoto N">N Sugimoto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chin, K" uniqKey="Chin K">K Chin</name></author><author><name sortKey="Sharp, Ka" uniqKey="Sharp K">KA Sharp</name></author><author><name sortKey="Honig, B" uniqKey="Honig B">B Honig</name></author><author><name sortKey="Pyle, Am" uniqKey="Pyle A">AM Pyle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kankia, Bi" uniqKey="Kankia B">BI Kankia</name></author><author><name sortKey="Marky, La" uniqKey="Marky L">LA Marky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jia, Z" uniqKey="Jia Z">Z Jia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Levin, Mk" uniqKey="Levin M">MK Levin</name></author><author><name sortKey="Gurjar, Mm" uniqKey="Gurjar M">MM Gurjar</name></author><author><name sortKey="Patel, Ss" uniqKey="Patel S">SS Patel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Appleby, Tc" uniqKey="Appleby T">TC Appleby</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Jang, Kyoung Jin" sort="Jang, Kyoung Jin" uniqKey="Jang K" first="Kyoung-Jin" last="Jang">Kyoung-Jin Jang</name></noRegion><name sortKey="Jang, Kyoung Jin" sort="Jang, Kyoung Jin" uniqKey="Jang K" first="Kyoung-Jin" last="Jang">Kyoung-Jin Jang</name><name sortKey="Jeong, Seonghwan" sort="Jeong, Seonghwan" uniqKey="Jeong S" first="Seonghwan" last="Jeong">Seonghwan Jeong</name><name sortKey="Kang, Dong Young" sort="Kang, Dong Young" uniqKey="Kang D" first="Dong Young" last="Kang">Dong Young Kang</name><name sortKey="Kim, Dong Eun" sort="Kim, Dong Eun" uniqKey="Kim D" first="Dong-Eun" last="Kim">Dong-Eun Kim</name><name sortKey="Sp, Nipin" sort="Sp, Nipin" uniqKey="Sp N" first="Nipin" last="Sp">Nipin Sp</name><name sortKey="Yang, Young Mok" sort="Yang, Young Mok" uniqKey="Yang Y" first="Young Mok" last="Yang">Young Mok Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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